Overall Categorization
Attributes of FASTQ
Alphabet Frequency & GC content
| Name | Value |
|---|---|
| InputFile | worm_PE620.fastq |
| #Read | 1,000,000 |
| #Base | 251,909,351 |
| AvgReadLen | 251.91 |
| MinReadLen | 35 |
| MaxReadLen | 301 |
| Name | Count | Freq% |
|---|---|---|
| A | 85,858,977 | 34.08% |
| C | 40,082,676 | 15.91% |
| G | 39,703,922 | 15.76% |
| T | 86,257,285 | 34.24% |
| N | 6,491 | 0.00% |
| GC% | - | 31.67% |
| Name | AreaFreq |
|---|---|
| Q30 & above | 87.6% |
| Q20-Q29 | 5.2% |
| Q15-Q19 | 3.8% |
| < Q15 | 3.4% |
| Name | AreaFreq |
|---|---|
| % of reads whose bases are all Q20 & above | 3.2% |
| % of reads whose bases are all Q15 & above | 6.9% |
| % of reads whose bases are all Q10 & above | 99.8% |
| Name | Coverage of reads | Remark |
|---|---|---|
| % of High-quality reads Coverage of reads that 100% of their bases with Q20 & above | 3.2% | %HighQ(20) >= 100% (i.e., MinimaQ>=20) |
| Coverage of reads that >= 95% of their bases with Q20 & above | 57.7% | %HighQ(20) >= 95% |
| Coverage of reads that >= 90% of their bases with Q20 & above | 77.6% | %HighQ(20) >= 90% |
| Coverage of reads that >= 90% of their bases with Q15 & above | 96.9% | %HighQ(15) >= 90% |
| % of Poor-quality reads Coverage of reads that > 10% of their bases with Q14 & less | 3.1% | 1 - {%HighQ(15) >= 90%} |
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