Pre-Assembly SQUAT Report
Wed Apr 25 19:48:21 2018

Summary

Overall Categorization
Attributes of FASTQ
Alphabet Frequency & GC content
Distribution of Bases' Quality Values
Distribution of Reads' MinimaQ Values
Covergae of Reads with Sufficient High-Quality Bases






Notes: The report requires Internet connection to show the interactive charts of distribuions made by Google chart.

worm_PE620


Attributes of FASTQ

NameValue
InputFileworm_PE620.fastq
#Read1,000,000
#Base251,909,351
AvgReadLen251.91
MinReadLen35
MaxReadLen301



Alphabet Frequency & GC content

NameCountFreq%
A85,858,97734.08%
C40,082,67615.91%
G39,703,92215.76%
T86,257,28534.24%
N6,4910.00%
GC%-31.67%



Distribution of Bases' Quality Values

NameAreaFreq
Q30 & above87.6%
Q20-Q295.2%
Q15-Q193.8%
< Q153.4%



Distribution of Reads' MinimalQ Values

NameAreaFreq
% of reads whose bases are all Q20 & above3.2%
% of reads whose bases are all Q15 & above6.9%
% of reads whose bases are all Q10 & above99.8%



Coverage of Reads with Sufficient High-Quality Bases

NameCoverage of readsRemark
% of High-quality reads
Coverage of reads that 100% of their bases with Q20 & above
3.2%%HighQ(20) >= 100%
(i.e., MinimaQ>=20)
Coverage of reads that >= 95% of their bases with Q20 & above57.7%%HighQ(20) >= 95%
Coverage of reads that >= 90% of their bases with Q20 & above77.6%%HighQ(20) >= 90%
Coverage of reads that >= 90% of their bases with Q15 & above96.9%%HighQ(15) >= 90%
% of Poor-quality reads
Coverage of reads that > 10% of their bases with Q14 & less
3.1%1 - {%HighQ(15) >= 90%}
%HighQ(q)